All data are presented as the mean ± SEM. Comparisons between two groups were performed using the non-parametric test Mann Whitney and the growth curve was analyzed by repeated measures Two-way ANOVA. For explant cultures, results from dual immunohistochemistry (IHC) experiments (e.g. NF/GFP) were analyzed by two-way ANOVA with Bonferroni correction for multiple comparisons, and the results of singular IHC (AgRP) were analyzed by one-way ANOVA with Newman Keuls’ multiple comparison test. Statistically non significant results were abbreviated “NS” in text. Statistically significant results are marked with asterisks, * p < 0.05; ** p < 0.01; *** p < 0.001.
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