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SIRT3 enzymatic activity was assayed with the Fluorogenic SIRT3 assay kit (BPS Bioscience, CA, USA) following the manufacturer’s instructions, with slight modifications. Mitochondrial protein (1 μg) was incubated at 37°C for 45 min with specific substrates; 25 μl of developer was added; and after 45 min of incubation, SIRT3 activity was measured using a fluorimetric microplate reader at 360 nm/460 nm.
Copyright and License information: ©2017 Lee et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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