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Human umbilical vein endothelial cell (HUVEC) culture
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Primary HUVECs and EC growth medium were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA). Culture flasks were coated with poly-l-lysine before use, and the cells were cultured in EC supplemented with EC growth supplement, 5% FBS and penicillin/streptomycin solution at 37°C in 5% CO2. HUVECS in the 2nd to 5th passages were used in all experiments.
Copyright and License information: ©2017 Zhou et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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