Measurement of cytokine production

YW Ya-xian Wu
HH Hui-qiong He
YN Yun-juan Nie
YD Yun-he Ding
LS Lei Sun
FQ Feng Qian
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Frozen lungs (right upper lobe) were homogenized and the supernatant was collected to measure the expression of cytokines TNF-α and IL-6 using ELISA assay kits. These concentrations were interpolated from the standard curves for recombinant TNF-α and IL-6.

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