in vitro cell migration/invasion assays

Transwell assays were performed to assess the cell migration and invasion ability using BD BioCoat Control Cell Culture Inserts and BD BioCoat BD Matrigel™ Invasion Chambers, respectively [26]. Briefly, cells were seeded in the upper Boyden chambers of the cell culture inserts. After 24h of incubation, cells that adhered to the lower membrane were stained with DAPI in the dark, imaged and counted. Three random fields were captured at 200× magnification under a microscope. The number of cells on the lower surface was compared among the groups.