Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Fluorescence microscopy.

GH Gisela von Hoven
AR Amable J. Rivas
CN Claudia Neukirch
MM Martina Meyenburg
QQ Qianqian Qin
SP Sapun Parekh
NH Nadja Hellmann
MH Matthias Husmann
request Request a Protocol
ask Ask a question
Favorite

Immunofluorescence analysis of ceramide and the lysosomal marker protein LAMP-1 was performed with MEF because the available antibodies yielded unspecific staining in HaCaT cells. The staining protocols for LAMP-1 and ceramide were as described in the supplemental material.

Copyright and License information:  ©2017 von Hoven et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A