Agarose Gel Electrophoresis.

Annealed samples were subjected to gel electrophoresis in 1% Tris-borate-EDTA buffer including 11 mM MgCl₂, at 70 V for 3 h in an ice-water bath. Gels were stained with SyberR Safe before imaging. Bands corresponding to the correctly folded structures were then visualized with UV light and cut out from the gel. Excised bands were crushed and transferred into DNA gel extraction spin column (BIO-RAD, catalog number: 732–6166). The DNA structure solution was recovered by centrifugation of the loaded column for 10 min at 10,000 × g.