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Adenosine concentrations were measured using a high-performance liquid chromatography (HPLC) system equipped with an Inertsil ODS-SP C18 chromatogram column (4.6 mm × 250 mm, 5 µm, Japan) using a mobile phase consisting of acetonitrile and 0.04 M potassium dihydrogen phosphate at the volume ratio of 5: 95. Identification and quantification of adenosine peaks were done by comparison to retention times of known standards and peak integration and normalization.
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