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The cells were cultured at 60–80% confluence in 24-well plates for 12 h. The reporter plasmids and miR-486-5p were transiently transfected into the cells. After 48 h, the Dual-Luciferase reporter assay (Promega, Madison, WI, USA) was used to measure the Renilla luciferase activity and the data were normalized with firefly luciferase.
Copyright and License information: ©2017 Lang et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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