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Reactive oxygen species (ROS) assay
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The dichlorofluorescin diacetate (DCFH-DA) fluorescent dye (Invitrogen) was employed to quantify cellular ROS intensity [25]. In brief, 1 μM of DCFH-DA were added to the cultured spinal cord neurons for 45 min. Neurons were then washedS, and subjected to examination of fluorescence intensity under a Fluorescence Microplate Reader (Synergy 2, BioTek, Winooski, VT). ROS intensity OD was expressed as fold change of untreated control group.
Copyright and License information: ©2017 Xu et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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