Acridine orange staining for acidic vesicular organelles

MC Mingbo Cai
SJ Shan Jin
LD Lu Deng
LZ Liancheng Zhu
ZH Zhenhua Hu
DL Dawo Liu
JL Juanjuan Liu
MT Mingzi Tan
JG Jian Gao
HW Huimin Wang
BL Bei Lin
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Acridine orange was added at a final concentration of 1 μg/mL for 15 min, and then washed with PBS. Images were obtained using a fluorescence microscope (Olympus) equipped with a digital camera (Olympus).

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