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Experimental DM was induced in overnight fasted rats by intraperitoneal injection of STZ (Sigma- Aldrich Corp, St. Louis, MO, USA) at a single dose of 60 mg/kg body weight dissolved in saline solution. After injection, the rats had free access to food and water. DM was allowed to develop and stabilize in these STZ-treated rats over a period of four days. DM was defined in these rats using determination of fasting blood glucose levels. The blood glucose levels over than 17 mmol/L were considered as diabetic model rats.
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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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