Blood samples (1 ml) were collected from the heart following completion of treatment, and the sera were separated by centrifugation at 3,000 × g for 5 min at 4°C. Concentrations of total serum IgE were measured using a Mouse IgE ELISA MAX™ Deluxe kit (cat. no. 432406) purchased from BioLegend. Inc. (San Diego, CA, USA), according to the manufacturer's protocols.
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