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20 μL of purified protein solutions reacted with 1% sodium alginate at a ratio of 1:1 (v/v) for 24 h were mixed with equal volumes of DNS reagent [20]. DNS reagent was prepared by diluting 5 mg of 3,5-dinitrosalicylic acid and 16 mg potassium sodium tartrate tetrahydrate in 1 mL of 0.4 M NaOH. The DNS reagent can be scaled up accordingly. Mixtures were boiled at 100°C for 10 min, cooled to room temperature for 2 min and absorbance of generated products were measured at 530 nm. All reactions were conducted using 1 μg/μL (final concentration) of purified protein.
Copyright and License information: ©2016 Mori et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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