Reagents

Acrylamide, tris(hydroxymethyl)aminomethane, NADPH, ammonium persulfate, bovine serum albumin, ethyleneglycoltetraacetic acid (EGTA), poly[dI-dC], spermidine, dithiothreitol (DTT), phenylmethanesulfonylfluoride (PMSF), naphthol AS-MX phosphate, Fast Red Salt, Ponceau S, an anti-mouse biotinylated antibody, ExtrAvidin-Alkaline Phosphatase, and menadione were purchased from Sigma−Aldrich (USA); Protan Nitrocellulose Transfer Membrane from Schleicher & Schuell (Germany); spermine from ICN (USA); ammonium sulfate from Helicon (Russia); caproic acid from AppliChem (Germany); N,N′-methylenebisacrylamide, sodium dodecyl sulfate (SDS), dimethyl sulfoxide (DMSO), HEPES, and TEMED from Serva GmbH (Germany); 2-mercaptoethanol and Tween 20 from Ferak Berlin (Germany); MgCl₂ from Janssen Chimica (Belgium); glycine and ethylenediaminetetraacetic acid (EDTA) from Merck (Germany); the TRI-Reagent and RNAsecure Reagent from Ambion, Inc. (USA); 7-ethoxyresorufin, 7-methoxyresorufin, M-MuLV reverse transcriptase, and RNasin from Promega Corporation (USA); the PCR kit qPCRmix-HS from Evrogen (Russia); glycerol from Panreac Quimica S.A.U. (Spain); α³²P-ATP from Amersham Pharmacia Biotech (USA); Klenow enzyme and sucrose from Medigen (Russia); oligonucleotides (primers) for analysis of the rat genes Cyp1a1, Cyp1a2, AhR, AhRR, ARNT, and Gapdh as well as XRE3, NRE (negative regulatory element), C/EBP, and random hexanucleotide primers were from Syntol (Russia) or Medigen (Russia). All other chemicals were of analytical grade.