2.9. Statistical analysis

Results were presented as means with the standard error of the mean. Values as percentage relative to the vehicle control were presented for most assays with exception of mRNA expression analysis and the GLUT4 translocation assay, which were presented as fold change. Relative results were arcsine transformed to ensure normality before any statistical analysis. Comparison of different levels of TPhP or DPhP with the vehicle control was evaluated with one-way ANOVA followed by a Dunnet’s multiple comparison test. Statistical analysis of effects of TPhP or DPhP (Factor 1), with or without either insulin or isoproterenol (Factor 2), and their interactions, was performed with two-way ANOVA followed of Dunnett’s multiple comparison test. The differences between groups were considered statistically significant if the adjusted P value was below 0.05 for the factors and below 0.1 for their interactions. All statistical analyses and graphs were executed with GraphPad Prism v7 (GraphPad Software, Inc., La Jolla, USA).