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The cytotoxicity of glecaprevir for Huh-7, HepG2, and MT4 cells was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma-Aldrich, St. Louis, MO) colorimetric assay. Huh-7 cells containing the genotype 1a replicon or HepG2 cells (4,000 cells/well) were plated in 96-well plates and incubated overnight before the addition of glecaprevir. For MT4 cells, 10,000 cells/well were plated immediately before the addition of glecaprevir. The rest of the assay was performed as described previously (33).
Copyright and License information: ©2017 Ng et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
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