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Luminometric-based assay (LUMA) for global DNA methylation

LL L. V. Laing
JV J. Viana
ED E. L. Dempster
MT M. Trznadel
LT L. A. Trunkfield
TW T. M. Uren Webster
RA R. van Aerle
GP G. C. Paull
RW R. J. Wilson
JM J. Mill
ES E. M. Santos
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The LUMA assay was performed as described by Karimi et al. (2006) using DNA extracted from gonad samples from 8 individual fish per treatment.67 Sufficient quantities of DNA were not available to perform the LUMA assay in liver samples; therefore, analysis of global DNA methylation were conducted only for gonad samples. Each DNA sample (250 ng) was digested in duplicate with HpaII and MspI, and data were normalized to the EcoRI peak to account for any technical differences between samples.68 Global DNA methylation values were calculated according to the formula [HpaII(G)/EcoRI(T)]/[MspI(G)/EcoRI(T)], where G and T refer to the peak heights for HpaII or MspI (methylation) and EcoRI (input DNA), respectively.

Copyright and License information:  ©2016 The Author(s). Published with license by Taylor & Francis Group, LLC
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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