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Surgical procedure

JW Jun Wang
WB Weina Bi
WZ Wei Zhao
MV Merina Varghese
RK Rick J. Koch
RW Ruth H. Walker
RC Roshantha A. Chandraratna
MS Martin E. Sanders
AJ Amanda Janesick
BB Bruce Blumberg
LW Libby Ward
LH Lap Ho
GP Giulio M. Pasinetti
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All surgical procedures were performed under general anesthesia with ketamine/xylazine. 2μl 6-OHDA (20 mg/ml in 0.02% ascorbic acid) was stereotaxically injected into the right striatum of male SD rats. Lesion coordinates were set according to bregma and dura in mm: ML-3.7; AP-1.0; DV-5.5. The injection rate was 2μl/5 min and the injecting needle was left for 2 minutes after injection to avoid back flow. Apomorphine-induced rotations (see below) were assessed three days post-lesion and rats with ≥ 30 full contralateral rotation/30 minutes were included in the study.

Copyright and License information:  ©2016 Wang et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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