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Cytokine and chemokine production in the supernatants of iMDDCs was evaluated using commercial ELISA kits following manufacturer’s instructions. The concentration of CCL3, CCL4, CCL5, IL1-β and IL-12 was measured using DuoSet kits (R&D Systems). The concentration of IL-6, TNFα, and GM-CSF was measured using Kit ELISA-Ready-Set-GO!® (Affimetrix eBioscience, San Diego, CA, USA).
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