High-content TFEB nuclear translocation assay

JS Ju-Xian Song
YS Yue-Ru Sun
IP Ivana Peluso
YZ Yu Zeng
XY Xing Yu
JL Jia-Hong Lu
ZX Zheng Xu
MW Ming-Zhong Wang
LL Liang-Feng Liu
YH Ying-Yu Huang
LC Lei-Lei Chen
SD Siva Sundara Kumar Durairajan
HZ Hong-Jie Zhang
BZ Bo Zhou
HZ Hong-Qi Zhang
AL Aiping Lu
AB Andrea Ballabio
DM Diego L. Medina
ZG Zhihong Guo
ML Min Li
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To quantify TFEB subcellular localization, a high-content assay was performed using stable HeLa cells overexpressing 3xFlag-TFEB according to our previous protocols.9

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