Query of TCGA and METABRIC breast cancer data sets.

We evaluated the publicly available TCGA and METABRIC breast cancer data accessible through the Cancer Genomics Data Server (CGDS; http://www.cbioportal.org/public-portal) hosted by the Computational Biology Center at Memorial-Sloan-Kettering Cancer Center through C-Bioportal for Cancer Genomics. We queried somatic mutations, copy number alterations, and mRNA expression for a panel of genes of interest, including ADHFE1, MYC, IDH2, IDH1, and PHGDH, in the TCGA dataset. We also downloaded METABRIC data for ADHFE1 copy number alterations and patient survival. A survival analysis was performed comparing patients with and without an ADHFE1 amplification in their tumors, using Kaplan-Meier and Cox regression modeling in the R survival package.