Hormonal Induction of Tyloses in Vitro

Plantlets grown in vitro with well-developed roots, typically 3 weeks old unless otherwise stated, were used for the hormone application experiments. The hormone stocks (0.2%, w/v or v/ v, in 2% DMSO [Duchefa Biochemie]), pH 5.6 adjusted with KOH, were filter sterilized. The filter-sterilized hormones were applied in volumes of approximately 2 mL to the sterile medium to give a final concentration of 100 μm unless otherwise stated. Control treatments consisted of 2% DMSO without hormones. JA and (−)-jasmonic acid methyl ester (MeJA) were from OlChemIm, and ACC was from Sigma-Aldrich.