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Briefly, hepatocytes were cultured in collagen-coated 96-well black plates with transparent bottoms and treated with paeonol at different concentrations in absence or presence of 5 mM APAP or 250 μM H2O2 for 6 h. The hepatocytes were subsequently loaded with 10 μM DCFH-DA for 30 min at 37°C. After washing twice with PBS. Fluorescence intensity in cells was detected at excitation and emission wavelengths of 490 nm and 520 nm using a fluorescence microplate reader (Molecular Devices, Sunnyvale, CA).
Copyright and License information: ©2016 Ding et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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