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Rats were anesthetized with 5% chloral hydrate (0.6 mL/kg) and decapitated 48 hours after TBI. The brains were quickly removed. The olfactory lobe and cerebellum were removed, and excess water was removed from the surface of the brain by absorbing with filter paper. The samples were immediately weighed to obtain the wet weight, then heated at 100°C for 24 hours (Lee et al., 2008) and weighed again to obtain the dry weight. Brain water content was calculated as a percentage using the following formula: (wet weight − dry weight)/wet weight × 100%.
Copyright and License information: ©2016 Neural Regeneration Research
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