Lectin blotting

Lectin blotting was performed according to a previously published procedure ²². Namely, purified IgG was separated in 10% SDS‐PAGE gels and subsequently transferred onto polyvinylidene difluoride (PVDF) membrane. The membrane was blocked with 3% BSA and then washed three times with 20 mmol/L Tris‐HCl (pH 7.5)‐0.15 mol/L NaCl‐0.05% Tween20 (TTBS). The membrane was incubated overnight at 4°C with 2 μg/mL AAL‐biotin. After washing three times with TTBS, the membrane was incubated with anti‐biotin‐horseradish peroxidase conjugate for 1 h. The membrane was extensively washed with TTBS and the reactivity was developed by ECL‐Plus (GE Healthcare) according to the manufacturer's protocol.