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ELISA

TN Tessa D. Nauta
MD Monique C. A. Duyndam
EW Ester M. Weijers
VH Victor M. W. van Hinsbergh
PK Pieter Koolwijk
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For enzyme-linked immunosorbent assay (ELISA) determination of soluble uPA and PAI-1 antigens, supernatants were collected from the 3D tube formation assay 72 hours after stimulation and assayed as previously described [24,36]. The monoclonal uPAR-blocking antibody H-2 came from Boehringer Mannheim, Penzberg, Germany [37] and was used to determine the overall internalization of the uPA:PAI-1 complex by blocking the uPA binding to uPAR, as previously described [26].

Copyright and License information:  ©2016 Nauta et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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