Chemicals and reagents

Antibody for CD200 (AF2724) and DuoSet ELISA kits for human TNFα (DY210), soluble TNFR1 (sTNFR1, DY225), INFγ (DY285), IL-4 (DY204), IL-6 (DY206), IL-8 (DY208), and IL-10 (DY217B) were purchased from R&D Systems (Minneapolis, MN). Antibody for CD200R (S-19, sc-323725) was purchased from Santa Cruz Biotechnology (San Diego, CA). A human CD200 siRNA SMARTpool (L-012181-00-0005) was purchased from GE Dharmacon (Lafayette, CO). Lipofectamine®RNAiMAX Transfection Reagent (Cat: 13778100) was from Invitrogen (Carlsbad, CA). Opti-MEM® I Reduced Serum Medium (Cat: 31985070) was purchased from Gibco Thermo Fisher Scientific (Grand Island, NY). Dulbecco’s modified Eagle’s medium (DMEM), Percoll, and protease inhibitors were from Sigma (St. Louis, MO). All other chemicals were from Sigma unless otherwise noted.