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Liver TAG measurement

HW Hong Yu Wang
CQ Chao Quan
CH Chunxiu Hu
BX Bingxian Xie
YD Yinan Du
LC Liang Chen
WY Wei Yang
LY Liu Yang
QC Qiaoli Chen
BS Bin Shen
BH Bian Hu
ZZ Zhihong Zheng
HZ Haibo Zhu
XH Xingxu Huang
GX Guowang Xu
SC Shuai Chen
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Liver TAG levels were determined as previously described (Norris et al., 2003). Briefly, liver chunks were taken from sacrificed rats and snap-frozen in liquid nitrogen before further analysis. Extraction of liver chunks was carried out in ethanolic KOH, and the resultant extracts were subsequently neutralized. Free glycerol resulted from breakdown of TAG was determined using the Free Glycerol Reagent (F6428, Sigma-Aldrich).

Copyright and License information:  ©2016-07-04 2016. Published by The Company of Biologists Ltd
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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