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To perform the cell survival assay, cells were collected after 24 h incubation with vehicle or dapagliflozin pretreatment in 30-min ischemia and surviving cells were counted with Trypan blue staining. The percentage survival was determined by quantization of the relative viable number of treated cells divided by the viable number of untreated cells.
Copyright and License information: ©2016 Chang et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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