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(i) Concentrations of antibiotics in serum in vivo.

DP D. Pförringer
AO A. Obermeier
MK M. Kiokekli
HB H. Büchner
SV S. Vogt
AS A. Stemberger
RB R. Burgkart
ML M. Lucke
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The availability of antibiotic ingredients in serum was tested by drawing venous blood from the ear of the animals and centrifuging it for 10 min at 3,500 × g (Varifuge 3.0R; Heraeus Sepatech, Germany). Serum samples were taken after 24, 48, and 72 h as well as after days 7, 14, 21, and 28 and stored at −20°C until analysis at the LC Labor Consult GmbH (Germany). An ADVIA 1650 Chemistry System (Siemens Healthcare Diagnostics, Germany) was used to determine antibiotic levels in serum for gentamicin, vancomycin, and tobramycin.

Copyright and License information:  ©2016, American Society for Microbiology. All Rights Reserved.

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