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B6 and HOD recipient mice were treated weekly with an intraperitoneal (i.p.) injection of 300 μg of anti-CD25 (BioXcell, clone PC-61) or anti-HRPN Rat IgG1 isotype control (BioXcell). In some experiments, recipient mice were immunized subcutaneously with 100 μg of ovalbumin (Sigma) emulsified in CFA (Difco Labs). Sera were collected 14 days post-treatment.
Copyright and License information: ©2016 Richards, Kapp, Wang, Howie and Hudson.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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