3.4. UPLC Condition

UPLC analysis was performed on a Shimadzu Nexera LC-30A (Shimadzu, Kyoto, Japan), including an autosampler and a quaternary solvent delivery system. Separation was performed using an InertSustainSwift^TM C18 column (2.1 mm × 150 mm, 1.9 μm, Shimadzu-GL, Tokyo, Japan). The mobile phases were acetonitrile (A) and 0.1% formic acid–water (B). A gradient elution was used: 10% A at 0–3 min, 10%–30% A at 3–8 min, 30%–50% A at 8–25 min, 50%–100% A at 25–32 min, and 100% A at 32–35 min. The mobile phase was established at a flow rate of 0.3 mL·min⁻¹, and the injection volume was 10 μL.