Neutrophils were harvested from the peritoneum of healthy WT and Gal3-/- mice, 8h after i.p. injection of 3% thioglycollate, 30 purified by magnetic cell sorting, and transferred [i.p. 2 x 106 cells per mouse] into Gal3-/- recipients at Day 4 of DSS treatment, as previously described.31
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