Virus, cells, and reagents

The JFH-1 strain of HCV was kindly provided by Takaji Wakita [40]. UV-inactivated virus was generated by exposing virus stocks to 2–3 minutes of UV light in a Stratalinker 3000 (Agilent Technologies). Huh7.5.1 cells were maintained in DMEM with 10% FBS, 100 U/mL penicillin/streptomycin, 2 mM L-glutamine, and 1% non-essential amino acids and infected with cell culture-derived JFH-1 at a multiplicity of infection (MOI) of 0.1. Huh7.5 cells harboring the HCV subgenomic replicon (Huh7.5-SG), a gift from Charles Rice, were cultured in Huh7.5.1 media in the presence of 750 μg/mL of G418 (Invivogen) to maintain viral RNA [41]. K1 and soraphen A were provided by Crop Solution, Inc. K1 was synthesized as described [42]. Sofosbuvir, the NS5B polymerase inhibitor (PSI-7977), was purchased from MedChem Express. The inhibitor of palmitoylation, 2-bromopalmitate (2-BP), was obtained from Sigma-Aldrich.