To understand the evolutional relationship between C. werkmanii BF-6 and 11 Citrobacter sp. strains, a phylogenetic tree was constructed using MEGA6 according to their 16S ribosomal RNA. For comparative genomic analysis of the BF-6 strain, genome sequences of these 11 Citrobacter sp. strains (Table (Table2)2) were downloaded from NCBI. The subprogram phyml of TreeBeST (http://treesoft.sourceforge.net/treebest.shtml) was used to construct a phylogenetic tree with the default parameters. Non-parametric bootstrap analysis with thousand re-samplings was conducted to obtain bootstrap values for all branches.
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