Stalled RNA polymerase II data (mapping of RNAs produced by promoter-proximal Pol II [35]) were downloaded from the GEO database, GSE18643 (samples GSM463298 and GSM463299).
Genome-wide data for insulator protein enrichment profiles were extracted from the modENCODE database (http://data.modencode.org/). In total, we used 16 tracks showing insulator protein distributions and 2 tracks showing enhancer localization [29]. The latter data were taken from S1 Table using Verification_status = “correct”, Positive = “1” tags. Data for the cis-regulatory modules (CRM) were downloaded from the RedFly resource (http://redfly.ccr.buffalo.edu/index.php) [36]. Description of the insulator protein and enhancer datasets is provided in S1 Table.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.