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Platelet calcium secretion measurement
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Platelet intracellular calcium flux was measured with a Fluo-8 calcium assay kit (AAT Bioquest). Fluo-8 solution and dye-loading solution was added to the platelet suspensions and platelet cells were incubated for 30 min at 37°C. The Ca2+ level was determined by monitoring the fluorescence intensity in Ex/Em = 490/525 nm with a fluorescence microplate reader (Glomax, Promega, USA).
Copyright and License information: ©2016 Cevik et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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