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Beta-lactamase activity was measured with an assay based on the chromogenic substrate nitrocefin (21) Briefly, 1 ml of culture was harvested and washed in buffer (pH 7.0). The cells were lysed in 1% Triton X-100, and the lysates were centrifuged. Beta-lactamase activity was determined as the rate of nitrocefin hydrolysis with protein as the normalization factor.
Copyright and License information: ©2016, American Society for Microbiology. All Rights Reserved.
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