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Whole blood cells were treated with LPS in the presence of of 0, 0.001, 0.01, 0.1 and 1.0 mg/ml Tyloxapol or 3% and 7% NaCl for 24 hrs at 37°C and 5% CO2. 7-Amino-actinomycin D (7-AAD) staining was used for the detection of dead cells. Therefore, samples were stained with 7AAD solution (Calbiochem, San Diego, CA) at 4°C protected from light for 20 minutes and analysed by flow cytometry (FACSVerse, Becton Dickinson, San Jose, CA, USA).
Copyright and License information: ©2016 Koppitz et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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