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Characterization of PMQR Bacteria and Their Gene Determinants
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Total DNA was extracted using a simple boiling method. The qnrA, qnrB, and qnrS genes were screened using a multiplex PCR method as described previously [18]. The genes qnrC, qnrD, aac(6′)-Ib-cr, and qepA were also detected [19]. The 16S rRNA, gyrB, and ropD genes were amplified and sequenced for phylogenetic analysis [20].
Copyright and License information: ©2016 Wen et al
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