Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Histological analysis for renal fibrosis

KS Kai Song
DS Deborah Stuart
NA Nikita Abraham
FW Fei Wang
SW Shuping Wang
TY Tianxin Yang
CS Curt D. Sigmund
DK Donald E. Kohan
NR Nirupama Ramkumar
request Request a Protocol
ask Ask a question
Favorite

Whole kidneys were fixed in 10% formalin, embedded in paraffin and 4 μm sections were obtained. Kidney sections were rehydrated with xylene and ethanol and stained with Masson Trichrome stain or Picro-Sirius solution (Electron Microscopy Sciences, Hatfield, PA). Renal collagen was determined as blue staining (Masson trichrome staining) or red staining (Picro-Sirius staining) under light microscopy. The area of renal fibrosis was calculated by 10 randomly selected non-overlapping fields at 200×magnification using NIH Image J software (National Institutes of Health, Bethesda, MD).

Copyright and License information:  ©2016 Song et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A