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[3H]-thymidine incorporation assay for cell proliferation
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1 × 104 cells plated onto each well of 6-well plates were incubated with 3 μCi/well of [methyl-3H]-thymidine (PerkinElmer Life Sciences) for 72 h. After washing 3 times with cold 1x PBS, cells were dissolved in 0.1 M NaOH. Incorporation of [3H]-thymidine was measured with a liquid scintillation counter (LS6000TA, Beckman Coulter).
Copyright and License information: ©2016 Chua et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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