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Phenotypes of transgenic plants for each construct were verified in at least three independent lines. Flowers were photographed using a Stereo Discovery V8 dissecting microscope (Carl Zeiss MicroImaging) with a Spot Flex digital camera (Diagnostic Instruments). To determine pollen viability, stage 12 anthers (Sanders et al., 1999) were collected and stained with the Alexander solution (Alexander, 1969) and photographed under an AXIO Scope A1 microscope (Carl Zeiss MicroImaging) with an Axio Cam HRc camera (Carl Zeiss MicroImaging).
Copyright and License information: ©2016 American Society of Plant Biologists. All rights reserved.
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