Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Liposome (2 mM total lipids) compositions are indicated in the figures or legends. Liposomes were incubated with 10 μM proteins (±Ca2+) in buffer A (unless stated otherwise) for 40 min at room temperature. The liposomes and bound proteins were isolated by flotation on a Histodenz (Sigma Aldrich) density gradients (40%:30%) using a SW 55 Ti rotor (Beckman Coulter; Boulevard Brea, CA) at 163,000 ×g for 40 min. Samples from the top and the bottom of the gradient (20 μl) were taken and analyzed by SDS-PAGE and Coomassie Brilliant Blue (CBB) staining.
Copyright and License information: ©2016, Wang et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
