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Heterokaryon Assays

JH Jens Heller
JZ Jiuhai Zhao
GR Gabriel Rosenfield
DK David J. Kowbel
PG Pierre Gladieux
NG N. Louise Glass
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Conidia of strains bearing different auxotrophic markers (his-3, ad-3B, or pyr-4; S4 Table) were harvested as described above. The conidial titer of one strain was adjusted to 3 x 106 conidia/ml, and the conidial titer of the forced communication partner (bearing a different auxotrophic marker) was adjusted to 3 x 105 conidia/ml. A 150 μl spore suspension of both strains was mixed and spread on modified VMM agar that promotes colonial growth (FGSC, http://www.fgsc.net/Neurospora/NeurosporaProtocolGuide.htm). Due to the complementing auxotrophic markers, only heterokaryotic, prototrophic fusion products were able to grow on VMM. Plates were incubated at 30°C for 4 d, when cell-forming units/plate were documented.

Copyright and License information:  ©2016 Heller et al
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