Cell Viability (MTT) Assay

Cell viability was assessed by MTT reduction assay [24]. At indicated time points of incubation, 200 μl aliquots were removed from cell suspensions into the 96-well microplates (1 × 10⁵/well), 20 μl of MTT solution (2,5 μg ml⁻¹) was added, and the plates were incubated for another 2 h. The culture medium was then replaced with 100 μl of DMSO. Diformazan formation was determined by measuring absorption at 570 nm with a plate reader (μQuant, BioTek, USA).