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Standard Western blot assays were used to analyze protein expression as we previously described [52]. Following antibodies were used: anti-MDM2 (2A10; generous gift from Dr. Arnold Levine), anti-p53 (DO-1, Santa Cruz Biotechonology), anti-p21 (Ab-1, EMD Millipore), anti-Puma (Cell Signaling), anti-Bax (Santa Cruz Biotechonology), and anti-actin (Sigma). The band intensity on Western blots was quantified by digitalization of the X-ray film and analyzed with Image J software (NIH, Bethesda, MD, USA) and normalized to Actin.
Copyright and License information: ©2016 Zhang et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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