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On the day of isolation, islets were picked into 100 μL of islet medium in each well of a 96-well V-bottom tissue culture-coated plate (catalog number 3894; Corning Life Sciences) using a P-20 micropipettor according to a protocol optimized in our laboratory (27). Insulin ELISAs were performed essentially as in Ref. 28.
Copyright and License information: ©2016 by the Endocrine Society
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