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PP2A Immunoprecipitation Phosphatase Assay Kit (Millipore) was used for immunoprecipitation and enzyme activity quantification. One reaction contained PP2A from sonicated cell lysate (aliquot corresponding to 2.5×105 cells). Released phosphate amounts were measured by a malachite green colorimetric assay. Experiments were performed 4 times with 2 technical replicates.
Copyright and License information: ©2016 The Author(s). Published with license by Taylor & Francis Group, LLC
This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
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